Coding

Part:BBa_K4378006:Design

Designed by: Sigurður Smári Davíðsson   Group: iGEM22_Uppsala   (2022-10-06)

TphA3, the ß-subunit of TPADO


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 148
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

During the design of this part, no illegal restriction sites had to be mutated out in silico prior to synthesis. This part has been codon optimized for E. coli.

Source

Sequence originates from Burkholderia xenovorans, the sequence published here was adapted from the following publication:

Bains J, Wulff JE, Boulanger MJ. 2012. Investigating Terephthalate Biodegradation: Structural Characterization of a Putative Decarboxylating cis-Dihydrodiol Dehydrogenase. Journal of Molecular Biology 423: 284–293.